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Image Search Results
Journal: bioRxiv
Article Title: A Ubiquitin-Binding Domain that Does Not Bind Ubiquitin
doi: 10.1101/375832
Figure Lengend Snippet: A . Diagram of sequential affinity purifications performed to reconstitute the SMARCAD1-KAP1 complex in vitro . B . Representative gel of the SMARCAD1-KAP1 complex reconstitution protocol. The final reconstituted SMARCAD1-KAP1 complex is pure and comprised of near-stoichiometric quantities of each protein. C . Analytical gel filtration chromatography confirming that SMARCAD1 (1.) and KAP1 (2.) elute as single peaks, while SMARCAD1 and KAP1 of the reconstituted SMARCAD1-KAP1 complex (3.) precisely co-elute in earlier eluting fractions.
Article Snippet: The eluate was concentrated to a volume of approximately 4mL using an Amicon Ultra-15 30K MWCO spin concentrator, before being loaded onto a 120mL HiPrep 16/60 Sephacryl S-400 HR
Techniques: In Vitro, Filtration, Chromatography